Name:Ji-Shen Zheng
Address:School of Life Sciences, University of Science and Technology 

of China, 230027 Hefei, P. R. China
2002.9 - 2006.7Bachelor, Department of chemistry, University of Science and Technology of China
2006.9 - 2012.7Ph.D, Department of chemistry, University of Science and Technology of China
2012.7 - 2014.3Post-doctoral fellow, Center for life sciences, Tsinghua University
2014.3 - 2016.7Associate professor, High Magnetic Field Laboratory of the Chinese of Academy of Sciences
2016.7 - presentProfessor, School of Life Sciences, University of Science and Technology of China
My research interests focus on the chemical synthesis of membrane proteins and their regulation by exogenous molecules. Membrane proteins play critical roles in many important biological problems and diseases. We have developed a removable backbone modification (RBM) strategy, combined with protein chemical ligation techniques, to achieve the chemical synthesis of a series of functionalized membrane proteins (e.g. post-translational modification or isotope labeling membrane proteins). Furthermore, based on high-throughput screening technology, we are focusing on the functional evolution of D-peptide ligands composed of D-amino acids to discover new-type bioactive D-peptide molecules/prodrug molecules. In addtion, by using modern biochemistry and biophysical techniques, the structure-function mechanism and precise regulation of membrane protein function are studied. More than 30 papers were published as the corresponding author/ first-authors in high-quality journals such as Nature Commun, Nature Protocols, J Am Chem Soc, Angew Chem Int Ed, and Acc Chem Res.
1.Qu, Q.; Gao, S.; Wu, F. M.; Zhang, M. G.; Li, Y; Zhang, L. H., Donald Bierer, Tian, C. L.*, Zheng, J. S.*; Liu, L.*, Synthesis of disulfide surrogate peptides incorporating large-span surrogate bridges through a native chemical ligation-assisted diaminodiacid strategy. Angew. Chem. Int. Ed. 2020, 59, 10.1002/anie.201915358.
2.Huang, D. L.; Montigny, C.; Zheng, Y.; Beswick, V.; Li, Y.; Cao, X. X.; Barbot, T.; Jaxel, C.; Liang, J.; Tian, C. L.; Jamin, N.; Zheng, J. S.*. Chemical Synthesis of Native S-palmitoylated Membrane Proteins through Reversible Backbone Modification Assisted Ser/Thr Ligation. Angew. Chem. Int. Ed. 2020, 59, 10.1002/anie.201914836.
3.Zhang, B.; Deng, Q.; Zuo, C.; Yan, B.; Zuo, C.; Cao, X. X.; Zhu, T. F.; Zheng, J. S.*; Liu, L.*, Ligation of Soluble but Unreactive Peptide Segments in the Chemical Synthesis of Haemophilus Influenzae DNA Ligase. Angew. Chem. Int. Ed. 2019, 58, 12231-12237.
4.Qi, Y.-K.; Si, Y.-Y.; Du, S.-S.; Liang, J.; Wang, K.-W.*; Zheng, J. S.*, Recent advances in the chemical synthesis and semi-synthesis of poly-ubiquitin-based proteins and probes. Science China Chemistry 2019, 62 (3), 299-312.
5.Guo, Q. Y.; Zhang, L. H.; Zuo, C.; Huang, D. L.; Wang, Z. A.; Zheng, J. S.*; Tian, C. L.*, Channel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors. Protein Cell 2019, 10, 211-216.
6.Tang, S.; Liang, L. J.; Si, Y. Y.; Gao, S.; Wang, J. X.; Liang, J.; Mei, Z.; Zheng, J. S.*; Liu, L.* Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer. Angew. Chem. Int. Ed. 2017, 56, 13333-13337.
7.Tang, S.; Zuo, C.; Huang, D. L.; Cai, X. Y.; Zhang, L. H.; Tian, C. L.*; Zheng, J. S.*; Liu, L.* Chemical Synthesis of Membrane Proteins via the Removable Backbone Modification Method. Nature Protocols 2017, 12, 2554-2569.
8.Li, J. B.; Tang, S.; Zheng, J. S.*; Tian, C. L.*; Liu, L.* Removable Backbone Modification Method for the Chemical Synthesis of Membrane Proteins. Acc. Chem. Res. 2017, 50, 1143-1153.
9.Zheng, J.-S.; He, Y.; Zuo, C.; Cai, X. Y.; Tang, S.; Wang, Z. A.; Zhang, L.-H., Tian, C.-L.*; Liu, L.* Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification. J. Am. Chem. Soc. 2016, 138, 3553-3561.
10.Chen, X. Tang, S.; Zheng, J. S.; Zhao, R.; Wang, Z. P.; Shao, W.; Chang, H. N.; Cheng, J. Y.; Zhao, H.; Liu, L.*; Qi, H.* Chemical Synthesis of a Two-Photon-Activatable Chemokine and Photon-Guided Lymphocyte Migration in vivo. Nat. Commun. 2015, 6, 7220-7228.
11.Zheng, J.-S.; Yu, M.; Qi, Y.-K.; Tang, S.; Shen, F.; Wang, Z.-P.; Xiao, L.; Zhang, L.; Tian, C.-L.*; Liu, L.* Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry. J. Am. Chem. Soc. 2014, 136, 3695-3704.
12.Zheng, J.-S.; Tang, S.; Qi, Y.-K.; Wang, Z.-P.; Liu, L.* Chemical Synthesis of Proteins Using Peptide Hydrazides as Thioester Surrogates. Nat. Protoc. 2013, 8, 2483-2495.
13.Zheng, J.-S.; Tang, S.; Huang, Y.-C.; Liu, L.* Development of New Thioester Equivalents for Protein Chemical Synthesis. Acc. Chem. Res. 2013, 46, 2475–2484.
14.Zheng, J.-S.; Chang, H.-N.; Wang, F.-L.; Liu, L.* Fmoc Synthesis of Peptide Thioesters without Post-Chain-Assembly Manipulation. J. Am. Chem. Soc. 2011, 133, 11080-11083.

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