Name:Ji-Shen Zheng
Address:School of Life Sciences, University of Science and Technology 

of China, 230027 Hefei, P. R. China
2002.9 - 2006.7Bachelor, Department of chemistry, University of Science and Technology of China
2006.9 - 2012.7Ph.D, Department of chemistry, University of Science and Technology of China
2012.7 - 2014.3Post-doctoral fellow, Center for life sciences, Tsinghua University
2014.3 - 2016.7Associate professor, High Magnetic Field Laboratory of the Chinese of Academy of Sciences
2016.7 - presentProfessor, School of Life Sciences, University of Science and Technology of China
The main research is the “chemical synthesis and regulation of post-translational membrane proteins”. The post-translational modified membrane proteins are closely related to many important biological problems and diseases. We have developed a removable backbone modification (RBM) strategy, combined with protein chemical ligation technology, to achieve the in vitro chemical reconstitution of a series of biologically active small to medium-sized functionalized membrane proteins, such as site-specific post-translational modification or isotope labeling. Meanwhile, based on high-throughput screening technology, we are focusing on the functional evolution of natural protein ligand molecules to discover new active molecules/prodrug molecules. Finally, by using modern biochemistry and biophysical techniques, the structure-function mechanism and precise regulation of membrane protein function are studied. More than 30 papers were published as the first-author/corresponding authors in high-quality journals such as Nature Commun, Nature Protocols, J Am Chem Soc, Angew Chem Int Ed, and Acc Chem Res.
1.Guo, Q. Y.; Zhang, L. H.; Zuo, C.; Huang, D. L.; Wang, Z. A.; Zheng, J. S.*; Tian, C. L.* Channel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors. Protein Cell 2018.
2.Tang, S.; Liang, L. J.; Si, Y. Y.; Gao, S.; Wang, J. X.; Liang, J.; Mei, Z.; Zheng, J. S.*; Liu, L.* Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer. Angew. Chem. Int. Ed. 2017, 56, 13333-13337.
3.Tang, S.; Zuo, C.; Huang, D. L.; Cai, X. Y.; Zhang, L. H.; Tian, C. L.*; Zheng, J. S.*; Liu, L.* Chemical Synthesis of Membrane Proteins via the Removable Backbone Modification Method. Nature Protocols 2017, 12, 2554-2569.
4.Li, J. B.; Tang, S.; Zheng, J. S.*; Tian, C. L.*; Liu, L.* Removable Backbone Modification Method for the Chemical Synthesis of Membrane Proteins. Acc. Chem. Res. 2017, 50, 1143-1153.
5.Zheng, J.-S.; He, Y.; Zuo, C.; Cai, X. Y.; Tang, S.; Wang, Z. A.; Zhang, L.-H., Tian, C.-L.*; Liu, L.* Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification. J. Am. Chem. Soc. 2016, 138, 3553-3561.
6.Chen, X. Tang, S.; Zheng, J. S.; Zhao, R.; Wang, Z. P.; Shao, W.; Chang, H. N.; Cheng, J. Y.; Zhao, H.; Liu, L.*; Qi, H.* Chemical Synthesis of a Two-Photon-Activatable Chemokine and Photon-Guided Lymphocyte Migration in vivo. Nat. Commun. 2015, 6, 7220-7228.
7.Zheng, J.-S.; Yu, M.; Qi, Y.-K.; Tang, S.; Shen, F.; Wang, Z.-P.; Xiao, L.; Zhang, L.; Tian, C.-L.*; Liu, L.* Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry. J. Am. Chem. Soc. 2014, 136, 3695-3704.
8.Zheng, J.-S.; Tang, S.; Qi, Y.-K.; Wang, Z.-P.; Liu, L.* Chemical Synthesis of Proteins Using Peptide Hydrazides as Thioester Surrogates. Nat. Protoc. 2013, 8, 2483-2495.
9.Zheng, J.-S.; Tang, S.; Huang, Y.-C.; Liu, L.* Development of New Thioester Equivalents for Protein Chemical Synthesis. Acc. Chem. Res. 2013, 46, 2475–2484.
10.Zheng, J.-S.; Chang, H.-N.; Wang, F.-L.; Liu, L.* Fmoc Synthesis of Peptide Thioesters without Post-Chain-Assembly Manipulation. J. Am. Chem. Soc. 2011, 133, 11080-11083.

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