We are fosucsed on protein misfolding and aggregation that occur in stressed cells with deficient proteostasis. These molecular events have been associated with a variety of diseases that are termed as protein misfolding diseases. Protein aggregation is a multiple step process that involves misfolded soluble and insoluble aggregates. It is unclear which of these conformations could lead to cytotoxicity that is associated with diseases. In this seminar, I will describe how we have developed chemical methodologies that allow the community to visualize and differentiate, in live cells, the many conformations through the process of protein aggregation.